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Product Info Summary
| SKU: | M00199-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00199-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody catalog # M00199-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00199-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CEH-23
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Wilms Tumor Protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00199-1 is reactive to WT1 in Human, Mouse
Applications
M00199-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
103 kDa
Calculated molecular weight
49.188kDa
Background of WT1
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of WT1 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For WT1 (Source: Uniprot.org, NCBI)
Gene Name
WT1
Full Name
Wilms tumor protein
Weight
49.188kDa
Superfamily
EGR C2H2-type zinc-finger protein family
Alternative Names
AWT1; GUD; NPHS4GUD; WAGR; Wilms tumor 1; Wilms tumor protein; WIT-2; WT1; WT33 WT1 AWT1, GUD, NPHS4, WAGR, WIT-2, WT33 WT1 transcription factor Wilms tumor protein|Wilms tumor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WT1, check out the WT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody (M00199-1)
Hello CJ!
M00199-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response
Dusabimana T,Kim SR,Park EJ,Je J,Jeong K,Yun SP,Kim HJ,Kim H,Park SW.P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response.Mol Metab.2020 Dec;42:101089.doi:10.1016/j.molmet.2020.101089.Epub 2020 Sep 25.PMID:32987187;PMCID:PMC7568185.
Species: Mouse
M00199-1 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:NA
Dusabimana T,Park EJ,Je J,Jeong K,Yun SP,Kim HJ,Kim H,Park SW.Geniposide Improves Diabetic Nephropathy by Enhancing ULK1-Mediated Autophagy and Reducing Oxidative Stress through AMPK Activation.Int J Mol Sci.2021 Feb 6;22(4):1651.doi:10.3390/ijms22041651.
Species: Mouse
M00199-1 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:NA
Sun D, Bu L, Liu C, Yin Z, Zhou X, Li X, Xiao A. Plos One. 2013 May 28;8(5):E65042. Doi: 10.1371/Journal.Pone.0065042. Print 2013. Therapeutic Effects Of Human Amniotic Fluid-Derived Stem Cells On Renal Interstitial Fibrosis In A Murine Model Of U...
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Wilms Tumor Protein Rabbit Monoclonal antibody for IF for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
It shows on the product datasheet, M00199-1 anti-Wilms Tumor Protein Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-22
Question
I have a question about product M00199-1, anti-Wilms Tumor Protein Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00199-1 anti-Wilms Tumor Protein Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-11
Question
Will anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 work on feline IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Our lab technicians have not validated anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
I see that the anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-26
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1. Please let me know if you require anything else.
N. Singh
Verified customer
Asked: 2019-05-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-20
Question
Is a blocking peptide available for product anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1)?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
We do provide the blocking peptide for product anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-13
Question
We are currently using anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
The anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-19
